Generator

Part:BBa_K325229:Design

Designed by: Will Handley and Peter Emmrich   Group: iGEM10_Cambridge   (2010-10-23)


Green Firefly Luciferase and LRE
L. Cruciata
(E. coli optimised)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 3044
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 3278
    Illegal SpeI site found at 3044
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 2169
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 3044
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3044
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2118
    Illegal AgeI site found at 3820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1550
    Illegal SapI.rc site found at 3334


Design Notes

a


Source

a

References